Absorption and CD spectroscopy and modeling of various LH2 complexes from purple bacteria

Georgakopoulou, S. and Frese, R. N. and Johnson, E. and Koolhaas, C. and Cogdell, R. J. and van Grondelle, R. and van der Zwan, G.. (2002) Absorption and CD spectroscopy and modeling of various LH2 complexes from purple bacteria. Biophysical journal, Vol. 82, H. 4. pp. 2184-2197.

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Official URL: http://edoc.unibas.ch/dok/A5249167

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The absorption (OD) and circular dichroism (CD) spectra of LH2 complexes from various purple bacteria have been measured and modeled. Based on the lineshapes of the spectra we can sort the LH2 complexes into two distinguishable groups: "acidophila"-Iike (type 1) and "molischianum"-like (type 2). Starting from the known geometric structures of Rhodopseudomonas (Rps.) acidophila and Rhodospirillum (Rsp,) molischianum we can model the OD and CID spectra of all species by just slightly varying some key parameters: the interaction strength, the energy difference of alpha- and beta-bound B850 bacteriochlorophylls (BChls), the orientation of the B800 and B850 BChls, and the (in)homogeneous broadening. Although the ring size can vary, the data are consistent with all the LH2 complexes having basically very similar structures.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
UniBasel Contributors:Georgakopoulou, Sofia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Biophysical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:16

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