edoc

Identification of the upper exciton component of the B850 bacteriochlorophylls of the LH2 antenna complex, using a B800-free mutant of Rhodobacter sphaeroides

Koolhaas, M. H. C. and Frese, R. N. and Fowler, G. J. S. and Bibby, T. S. and Georgakopoulou, S. and van der Zwan, G. and Hunter, C. N. and van Grondelle, R.. (1998) Identification of the upper exciton component of the B850 bacteriochlorophylls of the LH2 antenna complex, using a B800-free mutant of Rhodobacter sphaeroides. Biochemistry, Vol. 37, H. 14. pp. 4693-4698.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5249166

Downloads: Statistics Overview

Abstract

In this paper, we report the circular dichroism (CD) spectra of two types of LH2-only mutants of Rhodobacter sphaeroides. In the first, only the wild type LH2 is present, while in the second, the B800 binding site of LH2 has been either destabilized or removed. For the first time, we have identified a band in the CD spectrum of LH2, located at similar to 780 nm, that can be ascribed to the high exciton component of the B850 band. The experimental spectra have been modeled by theoretical calculations. On this basis, the average interaction strength between monomers in the B850 ring can be estimated to be approximately 300 cm(-1). In addition, we suggest that in LH2 of Rb. sphaeroides the angles made by the Q(y) transitions of the B850 BChls with respect to the plane of the ring are slightly different from those calculated from the crystal structure of the Rhodopseudomonas acidophila LH2 complex.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
UniBasel Contributors:Georgakopoulou, Sofia
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:American Chemical Society
ISSN:0006-2960
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:16

Repository Staff Only: item control page