A method for nitrite removal in nitrate N and O isotope analyses

Granger, J. and Sigman, D. M. and Prokopenko, M. G. and Lehmann, M. F. and Tortell, P. D.. (2006) A method for nitrite removal in nitrate N and O isotope analyses. Limnology and Oceanography : Methods, Vol. 15, H. 4. pp. 205-212.

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Official URL: http://edoc.unibas.ch/dok/A5249289

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We describe a method to remove nitrite from seawater and freshwater samples to determine the nitrogen (N) and oxygen (O) isotopic composition of nitrate in aqueous samples (N-15/N-14 and O-18/O-16, respectively). This method is simple, inexpensive, and effective in the removal of nitrite without compromising the natural N and O isotopic composition of nitrate. Moreover, the method is nontoxic and compatible with the "denitrifier method" for nitrate N and O isotope analysis. Nitrite is removed from solution by reduction to nitric oxide ( NO) gas using ascorbic acid at a pH of similar to 3.5. The NO produced is continually degassed with an inert gas during the reaction, preventing O-2 from reacting with NO to form new nitrate. Nitrate N and O isotope ratios were measured with the denitrifier method. The precision of isotope measurements of nitrate by the denitrifier method after nitrite removal averaged +/- 0.34 parts per thousand for N-15/N-14 and +/- 0.39 parts per thousand for O-18/O-16.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Geowissenschaften > Aquatic and Isotope Biogeochemistry (Lehmann)
UniBasel Contributors:Lehmann, Moritz F
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society of Limnology and Oceanography
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:30
Deposited On:22 Mar 2012 14:17

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