(Strept)avidin as Host for Biotinylated Coordination Complexes:  Stability, Chiral Discrimination, and Cooperativity

Loosli, Andreas and Rusbandi, Untung Edy and Gradinaru, Julieta and Bernauer, Klaus and Schlaepfer, Carl Wilhelm and Meyer, Michel and Mazurek, Sylwester and Novic, Marjana and Ward, Thomas R.. (2006) (Strept)avidin as Host for Biotinylated Coordination Complexes:  Stability, Chiral Discrimination, and Cooperativity. Inorganic Chemistry, 45 (2). pp. 660-668.

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Official URL: http://edoc.unibas.ch/dok/A5254460

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Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)2(Biot-bpy)]2+ (1) in either avidin or streptavidin(strept)avidincan be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and Δ)-1 species were synthesized and incorporated in tetrameric (strept)avidin to afford (Δ-[Ru(bpy)2(Biot-bpy)]2+)x⊂avidin, (Λ-[Ru(bpy)2(Biot-bpy)]2+)x⊂avidin, (Δ-[Ru(bpy)2(Biot-bpy)]2+)x⊂streptavidin, and (Λ-[Ru(bpy)2(Biot-bpy)]2+)x⊂streptavidin (x = 1−4) For these four systems, the overall stability constants are log β4 = 28.6, 30.3, 36.2, and 36.4, respectively. Critical analysis of the CD titrations data suggests a strong cooperativity between the first and the second binding event (x = 1, 2) and a pronounced difference in affinity between avidin and streptavidin for the dicationic guest 1 as well as modest enantiodiscrimination properties with avidin as host.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Apr 2017 13:13
Deposited On:22 Mar 2012 14:06

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