Label-free protein assay based on a nanomechanical cantilever array

Arntz, Y. and Seelig, J. D. and Lang, H. P. and Zhang, J. and Hunziker, P. and Ramseyer, J. P. and Meyer, E. and Hegner, M. and Gerber, C.. (2003) Label-free protein assay based on a nanomechanical cantilever array. Nanotechnology, 14 (1). pp. 86-90.

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Official URL: http://edoc.unibas.ch/dok/A5262133

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We demonstrate continuous label-free detection of two cardiac biomarker proteins (creatin kinase and myoglobin) using an array of microfabricated cantilevers functionalized with covalently anchored anti-creatin kinase and anti-myoglobin antibodies. This method allows biomarker proteins to be detected via measurement of surface stress generated by antigen-antibody molecular recognition. Reference cantilevers are used to eliminate thermal drifts, undesired chemical reactions and turbulences from injections of liquids by calculating differential deflection signals with respect to sensor cantilevers. The sensitivity achieved for myoglobin detection is below 20 mug ml(-1). Both myoglobin and creatin kinase could be detected independently using cantilevers functionalized with the corresponding antibodies, in unspecitic protein background. This approach permits the use of up to seven different antigen-antibody reactions simultaneously, including an additional thermomechanical and chemical in situ reference. Applications lie in the field of early and rapid diagnosis of acute myocardial infarction.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)
UniBasel Contributors:Meyer, Ernst
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:IOP Publishing
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Nov 2017 12:46
Deposited On:22 Mar 2012 13:58

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