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Microsatellites for disentangling underground networks : strain-specific identification of Glomus intraradices, an arbuscular mycorrhizal fungus

Mathimaran, N. and Falquet, L. and Ineichen, K. and Picard, C. and Redecker, D. and Boller, T. and Wiemken, A.. (2008) Microsatellites for disentangling underground networks : strain-specific identification of Glomus intraradices, an arbuscular mycorrhizal fungus. Fungal genetics and biology, Vol. 45, H. 6. pp. 812-817.

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Official URL: http://edoc.unibas.ch/dok/A5249180

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Abstract

The underground network of arbuscular mycorrhizal (AM) fungi is decisive for the above-ground diversity of many plant ecosystems, but tools to investigate the population structure of AM fungi are sorely lacking. Here, we present a bioinformatics approach to identify microsatellite markers in the AM fungus Glomus intraradices. Based on 1958 contigs of this fungus, assembled from public databases, we identified 842 microsatellites. One hundred of them were subjected to closer scrutiny by designing flanking primers and performing an extensive screen to identify polymorphic loci. We obtained 18 polymorphic microsatellite markers, and we found that seven out of eight individual single-spore cultures of G. intraradices could readily be identified by at least five allelic differences, as compared to all other strains. Two single-spore cultures, however, nominally originating from completely different locations, displayed identity at all 18 loci, suggesting with 99.999999% probability that they represent a single clone.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Pflanzenphysiologie Zuckermetabolismus (Wiemken)
UniBasel Contributors:Natarajan, Mathimaran and Wiemken, Andres M. and Boller, Thomas and Redecker, Dirk and Ineichen, Kurt
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1087-1845
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:27
Deposited On:22 Mar 2012 13:57

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