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The Moraxella : IgD-binding protein MID/Hag is an oligomeric autotransporter

Hallström, Teresia and Müller, Shirley A. and Moergelin, Matthias and Möllenkvist, Andrea and Forsgren, Arne and Riesbeck, Kristian. (2008) The Moraxella : IgD-binding protein MID/Hag is an oligomeric autotransporter. Microbes and infection, Vol. 10, H. 4. pp. 374-381.

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Official URL: http://edoc.unibas.ch/dok/A5262462

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Abstract

The IgD (IgD)-binding protein MID/Hag of the human respiratory pathogen Moraxella catarrhalis is an outer membrane protein of approx. 200 kDa belonging to the autotransporter family. MID also functions as an adhesin and hemagglutinin. In the present paper, the ultrastructure of MID was mapped. Using a series of Escherichia coli transformants, the last 210 aa of the C-terminal region were shown to translocate protein MID through the outer membrane suggesting that MID has a beta -barrel structure comprising of 10 transmembrane beta -sheets. Electron microscopy mapping with gold-labeled specific antibodies, and partial unravelling using guanidine hydrochloride showed that the rest of the MID protein forms an approx. 120 nm long, fibrillar structure in which the individual monomers fold back on themselves to expose a globular distal domain at their tips comprising both the IgD-binding (MID962-1200) and adhesive (MID764-913) regions. This positions their N-termini close to the C-terminal membrane spanning domains. Mass measurements by scanning transmission electron microscopy (STEM) verified that the MID mol. is an oligomer. [on SciFinder (R)]
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Müller, Shirley
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1286-4579
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:26
Deposited On:22 Mar 2012 13:55

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