Jaiswal, Sumit. Characterization of early mouse mesenchymal stem/progenitor cells for generating cartilage and study their role in endochondral ossification. 2016, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_11725
A developmental paradigm was used to characterize the “stem” like properties of PαS subpopulations in vitro from postnatal day P2, since a significant increase of this population was observed from E18.5 until P2 after birth. The conditions of the in vitro trilineage differentiation and chondrogenic differentiation were refined to achieve better results by mimicking the molecular pathways operating during limb development. Two subsets of PαS cells were discriminated by the expression of CD90 marker (PαS+CD90+ and PαS+CD90- subsets). These subsets were assessed for their in vivo fate by engrafting cartilage templates generated from these cells subcutaneously in nude mice. Strikingly, the cartilage models generated from the PαS+CD90+ subset remodeled completely to undergo endochondral ossification and the resulting bone ossicles were invaded by host-derived bone marrow cells. On the other hand, the cartilage models generated from the PαS+CD90- subset appeared to remodel only partially. No endochondral ossification was observed, but the expression of Sox9 persisted, which pointed to maintenance of chondrogenic potential. As in vivo functions of MSCs may depend on their interaction with HSCs in the stem cell niche, cartilage templates generated from PαS subsets isolated at embryonic day E18.5 were also engrafted. This developmental stage coincides with migration of the first HSCs from fetal liver to the bone marrow. The remodeling of cartilage into bone was reduced and only small bone ossicles formed when cartilage templates from PαS+CD90+ cells were implanted.
Thus, this study focuses on extensive characterization of PαS cells to identify new subpopulations and refining the conditions of multilineage differentiation in these subpopulations with a developmental approach. Emergence of these subpopulations during mouse development to contribute towards cartilage and bone lineage was determined by studying in detail their in vivo fates during endochondral ossification.
|Advisors:||Zeller, Rolf and Martín, Iván|
|Faculties and Departments:||03 Faculty of Medicine > Departement Biomedizin > Division of Anatomy > Developmental Genetics (Zeller/Zuniga)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||1 Online-Ressource (149 Seiten)|
|Last Modified:||14 Sep 2016 11:19|
|Deposited On:||14 Sep 2016 11:11|
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