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Trimethylsilyl-O-methyloxime derivatives for the measurement of [6,6-2H2]-D-glucose-enriched plasma samples by gas chromatography-mass spectrometry

Kury, D. and Keller, U.. (1991) Trimethylsilyl-O-methyloxime derivatives for the measurement of [6,6-2H2]-D-glucose-enriched plasma samples by gas chromatography-mass spectrometry. Journal of chromatography, Vol. 572, H. 1-2. S. 302-306.

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Official URL: http://edoc.unibas.ch/dok/A6419831

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Abstract

A new method for the determination of the enrichment of [6,6-2H2]-D-glucose in human plasma by gas chromatography-mass spectrometry (GC-MS) is described. (2,3,4,5,6)-Pentakis-O-trimethylsilyl-O-methyloxime-D-glucose is used as a derivative for the GC measurement. Using GC-MS with electron-impact ionization, the enrichment is measured in the single-ion monitoring mode observing the masses m/z 319 and 321. In contrast to other methods the use of this glucose derivative reduced the amount of plasma needed from 200 to 10 microliters and no chemical ionization equipment is needed for the mass spectrometer.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Metabolism (Keller/Müller)
03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Ehemalige Einheiten Medizinische Fächer (Klinik) > Klinische Endokrinologie (Keller)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Ehemalige Einheiten Medizinische Fächer (Klinik) > Klinische Endokrinologie (Keller)
UniBasel Contributors:Keller, Ulrich O.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0021-9673
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:04 Sep 2015 14:32
Deposited On:04 Sep 2015 14:32

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