Li, Ying. Biochemical and clinical diagnostic aspects of circulating nucleic acids. 2005, PhD Thesis, University of Basel, Faculty of Medicine.
Official URL: http://edoc.unibas.ch/diss/DissB_7220
Several reports indicated that cell free fetal DAN can be detected in the urine of pregnant women. We attempted to reproduce those data. We examined the urinary DNA from normal pregnant women, as well as from pregnancies complicated by HELLP syndrome. In no instance were we able to detect fetal DNA in maternal urine. Our data suggest cell free fetal DNA is not readily detectable in maternal urine.
We also examined paternal RhD genotype by real time PCR assay since it is important to counsel a couple about the risk of HDN. We tested 39 samples obtained from males who had been serologically typed to be RhD. We designed two multiplex real-time quantitative PCR assays to simulataneously detect the RhD gene in relation to the SRY sequence and the GAPDH sequence, respectively. Our study showed the two assays were in complete concordance. Therefore, the result indicated that real time PCR can potentially be used for the determination of RhD zygosity.
|Committee Members:||Bickle, Thomas A. and Lindberg Gasser, Raija L.P.|
|Faculties and Departments:||03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Prenatal Medicine (Hahn)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||1|
|Last Modified:||30 Jun 2016 10:41|
|Deposited On:||13 Feb 2009 15:29|
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