Low, Shyan Huey. Genome-wide RNAi screen reveals host factors involved in "Brucella" infection. 2015, PhD Thesis, University of Basel, Faculty of Science.
Restricted to Repository staff only until June 2018.
Official URL: http://edoc.unibas.ch/diss/DissB_11306
Brucella is able to invade phagocytic and non-phagocytic cells and replicates in an intracellular compartment known as the Brucella-containing vacuole (BCV). Following entry into a host cell, the BCV traffics along the endocytic pathway and despite interacting with endo-lysosomal compartments, degradation in these compartments are avoided. At later stages of infection when intracellular proliferation occurs, the BCV is found in close association with the endoplasmic reticulum (ER). Despite many advances in the field, the molecular mechanisms on how Brucella enters cells, avoids lysosomal degradation, and finally establishes an intracellular niche remain largely unknown.
To study Brucella entry and replication in human cells, we performed a genome-wide, high-throughput microscopy-based RNA interference (RNAi) screen in HeLa cells. This allowed us to unravel known and novel host signaling pathways involved in Brucella infection. To dissect the stage of infection that is regulated by these signaling pathways, a high-throughput entry assay was developed to study early stages of Brucella infection. Follow up studies allow a deeper understanding of the role of the host signaling pathway of interest during Brucella infection.
|Committee Members:||Pieters, Jean|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Dehio)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||252 p.|
|Last Modified:||30 Jun 2016 10:57|
|Deposited On:||03 Sep 2015 09:24|
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