Itel, Fabian. Lateral diffusion processes in biomimetic polymer membranes. 2015, PhD Thesis, University of Basel, Faculty of Science.
Available under License CC BY-NC-ND (Attribution-NonCommercial-NoDerivatives).
Official URL: http://edoc.unibas.ch/diss/DissB_11280
In this thesis, the fluid properties of synthetic membranes composed of synthetic amphiphiles are studied based on a large library of block copolymers. These consist of poly(2-methyloxazoline) (PMOXA) and poly(dimethylsiloxane) (PDMS) and are used as diblock (PMOXA-b-PDMS, AB) and triblock (PMOXA-b-PDMS-b-PMOXA, ABA) copolymers. Variation of the molecular weight induces changes in the membrane thickness and thus the fluidity of the membrane. The diffusion of membrane proteins within synthetic triblock copolymer membranes was investigated. The study revealed that the membrane proteins are mobile even at hydrophobic mismatches of up to 7 nm, which is a factor of seven compared to mismatches existing in biological membranes. The advantage of PDMS-containing block copolymers is their enormous flexibility even at high molecular weights, which provides a similar membrane environment compared to biological phospholipid membranes. This explains and displays the ability of PDMS to compress in contact to membrane proteins. Their diffusion decreases steadily with increasing thickness mismatch.
The importance of a very flexible polymer for the generation of biomimetic membranes was elucidated for membrane protein insertion, such as PDMS, which offers high fluidity and high membrane stability within membranes with even large thicknesses. The properties of these synthetic membranes investigated here, i.e. fluidity, lateral diffusion and membrane thickness, are important for the generation of biomimetic membranes for technological applications.
|Advisors:||Meier, Wolfgang Peter|
|Committee Members:||Bruns, Nico|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)|
|Bibsysno:||Link to catalogue|
|Last Modified:||30 Jun 2016 10:57|
|Deposited On:||04 Aug 2015 14:48|
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