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Transposition of Tn951 (Tnlac) and cointegrate formation are thermosensitive processes

Cornelis, G.. (1980) Transposition of Tn951 (Tnlac) and cointegrate formation are thermosensitive processes. Journal of General Microbiology, Vol. 117, H. 1. S. 243-247.

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Official URL: http://edoc.unibas.ch/dok/A5259282

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Abstract

The frequency of transposition of Tnlac to pGC200, an IncFII R plasmid, increased during the storage of the host strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30 degrees C but it was nearly undetectable when the host strain was grown and kept at 37 degrees C. Fusions between two different plasmids carrying Tnlac with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tnlac.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Cornelis, Guy R.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Cambridge University Press
ISSN:0022-1287
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:22

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