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A new method for the physical and genetic mapping of large plasmids : application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium

Michiels, T. and Popoff, M. Y. and Durviaux, S. and Coynault, C. and Cornelis, G.. (1987) A new method for the physical and genetic mapping of large plasmids : application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium. Microbial pathogenesis, Vol. 3, H. 2. S. 109-116.

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Official URL: http://edoc.unibas.ch/dok/A5259266

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Abstract

A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Molecular Microbiology (Cornelis)
UniBasel Contributors:Cornelis, Guy R.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Academic Press
ISSN:0882-4010
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:20
Deposited On:22 Mar 2012 13:22

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