Bourgine, Paul. Combination of cell immortalization and apoptosis induction to engineer decellularized matrices as bone graft materials. 2013, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10662
The success of this approach requires a standard cell source capable of secreting an osteo-inductive ECM, but also the development of a suitable decellularization protocol that can lead to both an efficient cell removal from the graft and the preservation of ECM properties.
In this thesis, I report the combined development of a standardized cell source and an apoptotic-based decellularization strategy, through the generation of a death-inducible human Mesenchymal Stromal Cell (hMSC) line. This cell line was shown to retain typical hMSC properties while continuously proliferating without signs of tumorigenicity, and being efficiently inducible toward apoptosis. Using this unlimited and well-characterized cellular tool, we successfully generated acellular ECM-coated graft within a 3D perfusion bioreactor. In particular, the induction of cell apoptosis was used as a decellularization procedure leading to the better preservation of key ECM components, as compared to the conventional approach.
Overall, by relying on both a novel cell source and a new decellularization approach, the developed protocol may lead to the development of a bone repair treatment with superior standardization and possibly cost-effectiveness as compared to current strategies.
|Committee Members:||Affolter, Markus and Ehrbar, Martin|
|Faculties and Departments:||03 Faculty of Medicine > Bereich Operative Fächer (Klinik) > Querschnittsbereich Forschung > Tissue Engineering (Martin)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||113 S.|
|Last Modified:||30 Jun 2016 10:55|
|Deposited On:||17 Mar 2014 14:50|
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