Acetylation regulates the DNA end-trimming activity of DNA polymerase beta

Hasan, Sameez and El-Andaloussi, Nazim and Hardeland, Ulrike and Hassa, Paul O. and Bürki, Christine and Imhof, Ralph and Schär, Primo and Hottiger, Michael O.. (2002) Acetylation regulates the DNA end-trimming activity of DNA polymerase beta. Molecular cell, Vol. 10, H. 5. S. 1213-22.

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Official URL: http://edoc.unibas.ch/dok/A5839360

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We describe a novel regulatory mechanism for DNA polymerase beta (Polbeta), a protein involved in DNA base excision repair (BER). Polbeta colocalized in vivo and formed a complex with the transcriptional coactivator p300. p300 interacted with Polbeta through distinct domains and acetylated Polbeta in vitro. Polbeta acetylation was furthermore observed in vivo. Lysine 72 of Polbeta was identified as the main target for acetylation by p300. Interestingly, acetylated Polbeta showed a severely reduced ability to participate in a reconstituted BER assay. This was due to an impairment of the dRP-lyase activity of Polbeta. Acetylation of Polbeta thus acts as an intranuclear regulatory mechanism and implies that p300 plays a critical regulatory role in BER.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Molecular Genetics (Schär)
UniBasel Contributors:Schär, Primo Leo
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Cell Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2013 09:10
Deposited On:26 Apr 2013 06:57

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