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Determination of creatine and phosphocreatine in muscle biopsy samples by capillary electrophoresis with contactless conductivity detection

See, Hong Heng and Schmidt-Marzinkowski, Julia and Pormsila, Worapan and Morand, Réjane and Krähenbühl, Stephan and Hauser, Peter C.. (2012) Determination of creatine and phosphocreatine in muscle biopsy samples by capillary electrophoresis with contactless conductivity detection. Analytica Chimica Acta, 727. pp. 78-82.

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Official URL: http://edoc.unibas.ch/dok/A6070767

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Abstract

A capillary electrophoresis method with contactless conductivity detection was evaluated as a new approach for quantification of creatine and phosphocreatine in human quadriceps femoris biopsy samples. The running buffers employed consisted of 1 M acetic acid at a pH of 2.3 for the determination of creatine and 50 mM 3-(N-morpholino)propanesulfonic acid/30 mM histidine at a pH of 6.4 for the determination of phosphocreatine in the centrifuged muscle extracts. The limits of detection for creatine and phosphocreatine were found to be 2.5 and 1.0 mu M, respectively. Creatine and phosphocreatine were determined in six human muscle biopsy samples and the results were found comparable to those of a standard enzymatic assay. The procedures developed for creatine and phosphocreatine also allow the determination of creatinine as well as adenosine diphosphate and adenosine triphosphate.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Clinical Pharmacology (Krähenbühl)
05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Ehemalige Einheiten Pharmazie > Pharmakologie (Krähenbühl)
UniBasel Contributors:Hauser, Peter C. P. C. and Krähenbühl, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0003-2670
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Mar 2017 13:09
Deposited On:01 Mar 2013 11:09

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