Diefenbacher, Melanie. Analyses of vesicular transport within the endoplasmic reticulum-golgi interface in "Saccharomyces cerevisiae". 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10178
In this thesis, we used S. cerevisiae to examine the regulation of vesicular traffic at the ER-Golgi interface, more specifically the fusion of vesicles with ER membranes. The consumption of a vesicle at its target membrane is mediated by the orchestrated action of various members of conserved protein families that act in a regulated manner. Main players involved in vesicular fusion are Rab GTPases, tethering factors and SNAREs. The tethering factors and the Rab GTPases mediate the first contact of an incoming vesicle with its acceptor organelle, whereas the SNARE proteins are responsible for the final fusion event between vesicles and target membranes.
Here, we identified the Rab GTPase Ypt1p as mediator of vesicle fusion with the ER. Moreover, Ypt1p was not only required for vesicle fusion at the ER, but also for the maintenance of the morphology and protein composition of the Golgi, and for vesicle formation at the Golgi. In addition, the tethering complex responsible for the docking of Golgi-derived vesicles with the ER, the Dsl1 tethering complex was analyzed. We found that this complex, apart from mediating the first contact of the incoming vesicles with the ER membrane, seems to play an additional role in proofreading and stabilization of SNARE complexes that are responsible for vesicle fusion at the ER
|Committee Members:||Spiess, Martin|
|Faculties and Departments:||05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||84 S.|
|Last Modified:||30 Jun 2016 10:51|
|Deposited On:||27 Nov 2012 16:33|
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