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Specific impact of tobamovirus infection on the Arabidopsis small RNA profile

Hu, Q. A. and Hollunder, J. and Niehl, A. and Korner, C. J. and Gereige, D. and Windels, D. and Arnold, A. and Kuiper, M. and Vazquez, F. and Pooggin, M. and Heinlein, M.. (2011) Specific impact of tobamovirus infection on the Arabidopsis small RNA profile. PLoS one, Vol. 6, H. 5 , e19549.

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Official URL: http://edoc.unibas.ch/dok/A6001914

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Abstract

Tobamoviruses encode a silencing suppressor that binds small RNA (sRNA) duplexes in vitro and supposedly in vivo to counteract antiviral silencing. Here, we used sRNA deep-sequencing combined with transcriptome profiling to determine the global impact of tobamovirus infection on Arabidopsis sRNAs and their mRNA targets. We found that infection of Arabidopsis plants with Oilseed rape mosaic tobamovirus causes a global size-specific enrichment of miRNAs, ta-siRNAs, and other phased siRNAs. The observed patterns of sRNA enrichment suggest that in addition to a role of the viral silencing suppressor, the stabilization of sRNAs might also occur through association with unknown host effector complexes induced upon infection. Indeed, sRNA enrichment concerns primarily 21-nucleotide RNAs with a 5 '-terminal guanine. Interestingly, ORMV infection also leads to accumulation of novel miRNA-like sRNAs from miRNA precursors. Thus, in addition to canonical miRNAs and miRNA*s, miRNA precursors can encode additional sRNAs that may be functional under specific conditions like pathogen infection. Virus-induced sRNA enrichment does not correlate with defects in miRNA-dependent ta-siRNA biogenesis nor with global changes in the levels of mRNA and ta-siRNA targets suggesting that the enriched sRNAs may not be able to significantly contribute to the normal activity of pre-loaded RISC complexes. We conclude that tobamovirus infection induces the stabilization of a specific sRNA pool by yet unknown effector complexes. These complexes may sequester viral and host sRNAs to engage them in yet unknown mechanisms involved in plant: virus interactions.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanisches Institut > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Korner, Camilla Julie and Windels, David and Vazquez, Franck and Pooggin, Mikhail and Heinlein, Manfred and Hu, Quanan and Niehl, Annette
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:PubMed Central
ISSN:1932-6203
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:13 Sep 2013 07:57
Deposited On:08 Nov 2012 16:13

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