Towbin, Benjamin Daniel. Dynamics of subnuclear chromatin organization during "C. elegans" development : a role for H3K9 methylation. 2012, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_10089
In this thesis, I characterized a fluorescence based experimental system to monitor the perinuclear anchoring of heterochromatin during development of the nematode worm C. elegans. Using this system, I searched for the molecular factors needed to sequester silent chromatin at the nuclear periphery and identified two enzymes with histone methyltransferase activity. Both enzymes specifically methylate lysine 9 on histone H3 (H3K9). Interstingly, they do so in a step-wise manner: the first enzyme mono- and di-methylates H3K9. H3K9me1/2 is then used as a substrate for tri-methylation by the second enzyme.
The results shed light the previously uncharacterized mechanism of perinuclear chromatin sequestration and implicate a role for post-translational modifications of chromatin in its subnuclear organization.
|Committee Members:||Askjaer, Peter|
|Faculties and Departments:||03 Faculty of Medicine > Bereich Operative Fächer (Klinik) > Innere Organe > Urologie Kliniken BL (Gasser)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||87 S.|
|Last Modified:||30 Jun 2016 10:50|
|Deposited On:||22 Oct 2012 14:11|
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