edoc

Mutations of p34cdc2 phosphorylation sites induce premature mitotic events in HeLa cells : evidence for a double block to p34cdc2 kinase activation in vertebrates

Krek, W. and Nigg, E. A.. (1991) Mutations of p34cdc2 phosphorylation sites induce premature mitotic events in HeLa cells : evidence for a double block to p34cdc2 kinase activation in vertebrates. The EMBO journal, Vol. 10, H. 11. S. 3331-3341.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5249487

Downloads: Statistics Overview

Abstract

In vertebrates, entry into mitosis is accompanied by dephosphorylation of p34cdc2 kinase on threonine 14 (Thr14) and tyrosine 15 (Tyr15). To examine the role of these residues in controlling p34cdc2 kinase activation, and hence the onset of mitosis, we replaced Thr14 and/or Tyr15 by non-phosphorylatable residues and transfected wild-type and mutant chicken p34cdc2 cDNAs into HeLa cells. While expression of wild-type p34cdc2 did not interfere with normal cell cycle progression, p34cdc2 carrying mutations at both Thr14 and Tyr15 displayed increased histone H1 kinase activity and rapidly induced premature mitotic events, including chromosome condensation and lamina disassembly. No phenotype was observed in response to mutation of only Thr14, and although single-site mutation at Tyr15 did induce premature mitotic events, effects were partial and their onset was delayed. These results identify both Thr14 and Tyr15 as sites of negative regulation of vertebrate p34cdc2 kinase, and they suggest that dephosphorylation of p34cdc2 represents the rate-limiting step controlling entry of vertebrate cells into mitosis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:0261-4189
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:17

Repository Staff Only: item control page