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In vivo analysis of scaffold-associated regions in Drosophila : a synthetic high-affinity SAR binding protein suppresses position effect variegation

Girard, F. and Bello, B. and Laemmli, U. K. and Gehring, W. J.. (1998) In vivo analysis of scaffold-associated regions in Drosophila : a synthetic high-affinity SAR binding protein suppresses position effect variegation. The EMBO journal, Vol. 17, H. 7. S. 2079-2085.

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Official URL: http://edoc.unibas.ch/dok/A5257301

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Abstract

Scaffold-associated regions (SARs) were studied in Drosophila melanogaster by expressing a synthetic, high-affinity SAR-binding protein called MATH (multi-AT-hook), which consists of reiterated AT-hook peptide motifs; each motif is known to recognize a wide variety of short AT-rich sequences. MATH proteins were expressed specifically in the larval eye imaginal discs by means of the tetracycline-regulated transactivation system and tested for their effect on position effect variegation (PEV). MATH20, a highly potent SAR ligand consisting of 20 AT-hooks, was found to suppress whitemottled 4 variegation. This suppression required MATH20 expression at an early larval developmental stage. Our data suggest an involvement of the high AT-rich SARs in higher order chromatin structure and gene expression.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Gehring)
UniBasel Contributors:Gehring, Walter Jakob
Item Type:Article, refereed
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:0261-4189
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:19
Deposited On:22 Mar 2012 13:17

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