Thiéry, Odile. Molecular markers from the mitochondrial genome of arbuscular mycorrhizal fungi (Glomeromycota) : evolutionary dynamics and application. 2010, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9218
The respective region of the mtLSU was analyzed in five species of Glomus (G. mosseae, G. geosporum, G. caledonium, G. clarum, G. coronatum) from the same major clade (Glomus group A), Glomus sp. ISCB 34 from the related Glomus group B and two species of Scutellospora. The nucleotide polymorphism was very low among related morphospecies and differences were mainly caused by the exon/intron structure, limiting the resolution to a species level. Phylogenetic analyses suggested vertical inheritance from common ancestors for some introns as well as horizontal transfer for others.
Four mitochondrial intergenic spacers (rns/nad5, atp6/nad2, nad3/nad6, and nad6/cox3) were assessed as potential intraspecific markers. Combined, they offer a resolution even higher than the mtLSU, with the highest polymorphism found in the rns/nad5 spacer, most likely caused by processes of evolutionary dynamics involving homing endonucleases.
A mtLSU PCR approach was applied to study the population structure of G. intraradices at geothermal sites in Europe and North America, revealing considerable newly-discovered mtLSU sequence types as well as previously-reported types from arable fields. The molecular survey showed strong biogeographical structure in the occurrence of G. intraradices.
Overall, this PhD thesis project showed that the genetic characteristics of the AMF mitochondrion are promising for development of molecular genetic markers and evolutionary sequence analyses, and will therefore be relevant for further studies of genetics, ecology and evolution of AMF.
|Committee Members:||Redecker, Dirk and Boller, Thomas|
|Faculties and Departments:||05 Faculty of Science > Departement Umweltwissenschaften > Botanisches Institut > Pflanzenphysiologie Zuckermetabolismus (Wiemken)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||175 S.|
|Last Modified:||30 Jun 2016 10:41|
|Deposited On:||26 Jan 2011 16:17|
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