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Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation

Date Issued
2017-01-01
Author(s)
Keller, Sascha G.  
Pannwitz, Andrea  
Mallin, Hendrik  
Wenger, Oliver S.  
Ward, Thomas R.  
DOI
10.1002/chem.201703885
Abstract
Long-lived photo-driven charge separation is demonstrated by assembling a triad on a protein scaffold. For this purpose, a biotinylated triarylamine was added to a Ru II -streptavidin conjugate bearing a methyl viologen electron acceptor covalently linked to the N -terminus of streptavidin. To improve the rate and lifetime of the electron transfer, a negative patch consisting of up to three additional negatively charged amino acids was engineered through mutagenesis close to the biotin-binding pocket of streptavidin. Time-resolved laser spectroscopy revealed that the covalent attachment and the negative patch were beneficial for charge separation within the streptavidin hosted triad; the charge separated state was generated within the duration of the excitation laser pulse, and lifetimes up to 3120 ns could be achieved with the optimized supramolecular triad.
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