Ancient DNA sequence quality is independent of fish bone weight

Atmore, Lane M. and Ferrari, Giada and Martínez-García, Lourdes and van der Jagt, Inge and Blevis, Rachel and Granado, José and Häberle, Simone and Dierickx, Katrien and Quinlan, Liz M. and Lõugas, Lembi and Makowiecki, Daniel and Hufthammer, Anne Karin and Barrett, James H. and Star, Bastiaan. (2023) Ancient DNA sequence quality is independent of fish bone weight. Journal of archaeological science, 149. p. 105703.

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Official URL: https://edoc.unibas.ch/94427/

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The field of ancient DNA (aDNA) typically uses between 50 and 200 mg of minimum input weight of bone material for the extraction of DNA from archaeological remains. While laboratory and analysis techniques have focused on improved efficiency of extracting useable sequence data from older and poorer quality remains, bone material input requirements have rarely been critically evaluated. Here, we present the aDNA analysis of 121 size-constrained Atlantic herring remains - weighing between <10 and 70 mg - that were individually sequenced to explore the capacity of successful aDNA retrieval from small archaeological remains. We statistically evaluate the relationship between bone weight and several response variables, including library success, endogenous DNA content, and library complexity, i.e., the number of unique molecules that are obtained. Remarkably, we find no relationship between bone weight and library success, levels of endogenous DNA, or library complexity. Our results imply that - at least in the case of fish bone - even minute bones can yield positive results and that the presumed minimum sample size required should be re-evaluated. Archaeological site, instead of bone size, is the primary driver of DNA sequence quality. Our work expands the number of specimens considered suitable for aDNA analyses, and therefore facilitates efforts to minimize the destructive impact of aDNA research and mediate some of the ethical concerns surrounding destructive analysis. Keywords: Ancient DNA; Skeletal remains; Laboratory methods; Destructive analysis
Faculties and Departments:05 Faculty of Science
05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Archäozoologie (Schibler)
05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Integrative Prähistorische und Naturwissenschaftliche Archäologie (IPNA Schünemann)
UniBasel Contributors:Häberle, Simone
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Academic Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:02 May 2023 13:45
Deposited On:02 May 2023 13:45

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