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Molecular epidemiology of Mansonella perstans on Bioko Island: identification of risk factors, co-infection with malaria and Loa loa and impact in pregnant women

Yoboue, Aya Charlene. Molecular epidemiology of Mansonella perstans on Bioko Island: identification of risk factors, co-infection with malaria and Loa loa and impact in pregnant women. 2023, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: https://edoc.unibas.ch/94357/

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Abstract

Implementation of an effective and targeted mass drug administration program against filarial worms needs an accurate identification and mapping of the distribution of these parasites. Molecular diagnostic methods like polymerase chain reaction (PCR) based techniques have shown high sensitivity to detect and distinguish parasites at low infection levels and even in people that are amicrofilaremic by microscopy. However, larger scale implementation over time remains a challenge for low-income countries because of logistical complexity and costs, including collection, transportation and preservation of biological samples and running of the analytical tests.
In this study, we aimed to develop and implement a novel molecular based diagnostic approach for monitoring highly neglected filariasis causing parasites in Equatorial Guinea, West Africa. The ENAR protocol is an approach based on the use of nucleic acids (NA) extracted from dried blood retained on used malaria rapid diagnostic test (mRDT). Malaria RDTs used in this work were stored at room temperature for around 18 months before used for molecular analysis and served as reliable source for NA based detection of blood-dwellings pathogens beyond malaria. Our approach of repurposing used mRDTs for filarial parasites detection provides a versatile and cost-effective approach to monitor prevalence, genotype, infection intensity and co-infections of filarial nematodes and other blood borne infectious diseases.
Advisors:Daubenberger, Claudia
Committee Members:Utzinger, Jürg and Koudou, Guibehi Benjamin
Faculties and Departments:05 Faculty of Science
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Clinical Immunology (Daubenberger)
UniBasel Contributors:Daubenberger, Claudia and Utzinger, Jürg
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:15003
Thesis status:Complete
Number of Pages:1 Band (verschiedene Seitenzählungen)
Language:English
Identification Number:
  • urn: urn:nbn:ch:bel-bau-diss150039
edoc DOI:
Last Modified:05 May 2023 04:30
Deposited On:04 May 2023 07:27

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