Construction of cis-regulatory input functions of yeast promoters

Ratna, Prasuna and Becskei, Attila. (2011) Construction of cis-regulatory input functions of yeast promoters. In: Yeast Genetic Networks. Basel, pp. 45-61.

Full text not available from this repository.

Official URL: https://edoc.unibas.ch/94084/

Downloads: Statistics Overview


Promoters contain a large number of binding sites for transcriptional factors transmitting signals from a variety of cellular pathways. The promoter processes these input signals and sets the level of gene expression, the output of the gene. Here, we describe how to design genetic constructs and measure gene expression to deliver data suitable for quantitative analysis. Synthetic genetic constructs are well suited to precisely control and measure gene expression to construct cis-regulatory input functions. These functions can be used to predict gene expression based on signal intensities transmitted to activators and repressors in the gene regulatory region. Simple models of gene expression are presented for competitive and noncompetitive repressions. Complex phenomena, exemplified by synergistic silencing, are modeled by reaction-diffusion equations.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Synthetic Microbiology (Becskei)
UniBasel Contributors:Becskei, Attila
Item Type:Book Section, refereed
Book Section Subtype:Further Contribution in a Book
Publisher:Springer Nature
Series Name:Methods in Molecular Biology
Issue Number:734
Note:Publication type according to Uni Basel Research Database: Book item
Identification Number:
Last Modified:23 Mar 2023 08:01
Deposited On:22 Mar 2023 15:16

Repository Staff Only: item control page