Analysis of Tau/Nucleoporin Interactions by Surface Plasmon Resonance Spectroscopy

Diez, Lisa and Kapinos, Larisa E. and Lim, Roderick Y. H. and Wegmann, Susanne. (2023) Analysis of Tau/Nucleoporin Interactions by Surface Plasmon Resonance Spectroscopy. In: Protein Aggregation. New York, pp. 95-109.

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Tau, a soluble and predominantly neuronal protein, is best known for its microtubule (MT)-binding function in the cytosol, where it decisively contributes to stability as well as modulation of MT dynamics. In Alzheimer's disease and other tauopathies, Tau is altered into forming intracellular neurofibrillary tangles; additionally, also a mislocalization from the cytosol to the nucleus has been observed where interactions of Tau with the nucleus become possible. Using surface plasmon resonance (SPR), it was recently shown that Tau can directly interact with certain nucleoporins (e.g., Nup98), components of the nuclear pore complex (NPC). The NPC constitutes large regulated pores in the nuclear envelope that facilitate the bidirectional exchange of proteins, nucleic acids, and other biomolecules between the inner section of the nucleus and the cytosol, the nucleocytoplasmic transport. The mechanism of Tau/Nup interactions is as yet unknown, and a systematic interaction analysis of Tau with different Nups can be of high value to decipher the molecular binding mechanism of Tau to Nups. SPR is a useful tool to analyze binding affinities and kinetic parameters in a label-free environment. While one interaction partner is immobilized on a sensor chip, the second is supplied within a constant flow of buffer. Binding of mobile molecules to immobilized ones changes the refractive index of the medium close to the sensor surface with the signal being proportional to the bound mass. In this chapter, we describe the application of the SPR technique for the investigation of Tau binding to nucleoporins.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Kapinos Schneider, Larisa E. E and Lim, Roderick Y.H.
Item Type:Book Section, refereed
Book Section Subtype:Book Chapter
Series Name:Methods in Molecular Biology
Issue Number:2551
Note:Publication type according to Uni Basel Research Database: Book item
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Last Modified:01 Mar 2023 08:07
Deposited On:01 Mar 2023 08:07

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