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Special considerations for studies of extracellular vesicles from parasitic helminths: a community-led roadmap to increase rigour and reproducibility

White, R. and Sotillo, J. and Ancarola, M. E. and Borup, A. and Boysen, A. T. and Brindley, P. J. and Buzas, E. I. and Cavallero, S. and Chaiyadet, S. and Chalmers, I. W. and Cucher, M. A. and Dagenais, M. and Davis, C. N. and Devaney, E. and Duque-Correa, M. A. and Eichenberger, R. M. and Fontenla, S. and Gasan, T. A. and Hokke, C. H. and Kosanovic, M. and Kuipers, M. E. and Laha, T. and Loukas, A. and Maizels, R. M. and Marcilla, A. and Mazanec, H. and Morphew, R. M. and Neophytou, K. and Nguyen, L. T. and Nolte-'t Hoen, E. and Povelones, M. and Robinson, M. W. and Rojas, A. and Schabussova, I. and Smits, H. H. and Sungpradit, S. and Tritten, L. and Whitehead, B. and Zakeri, A. and Nejsum, P. and Buck, A. H. and Hoffmann, K. F.. (2023) Special considerations for studies of extracellular vesicles from parasitic helminths: a community-led roadmap to increase rigour and reproducibility. J Extracell Vesicles, 12. e12298.

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Abstract

Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Helminth Drug Development (Keiser)
UniBasel Contributors:Tritten, Lucienne
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2001-3078 (Electronic)2001-3078 (Linking)
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:23 Jan 2023 09:34
Deposited On:23 Jan 2023 09:34

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