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Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer

Lüönd, Fabiana and Santacroce, Natascha and Beisel, Christian and Guérard, Laurent and Bürglin, Thomas R. and Christofori, Gerhard and Sugiyama, Nami. (2022) Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer. STAR Protocols, 3 (2). p. 101438.

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Abstract

The various stages of epithelial-mesenchymal transition (EMT) generate phenotypically heterogeneous populations of cells. Here, we detail a dual recombinase lineage tracing system using a transgenic mouse model of metastatic breast cancer to trace and characterize breast cancer cells at different EMT stages. We describe analytical steps to label cancer cells at an early partial or a late full EMT state, followed by tracking their behavior in tumor slice cultures. We then characterize their transcriptome by five-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Luond et al. (2021).
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Imaging Core Facility (Biehlmaier)
UniBasel Contributors:Guerard, Laurent and Lüönd, Fabiana and Bürglin, Thomas and Christofori, Gerhard M. and Matsuda, Nami
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
e-ISSN:2666-1667
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:13 Dec 2022 14:21
Deposited On:13 Dec 2022 14:21

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