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Whole-genome characterisation of ESBL-producing E. coli isolated from drinking water and dog faeces from rural Andean households in Peru

Medina-Pizzali, M. L. and Venkatesh, A. and Riveros, M. and Cuicapuza, D. and Salmon-Mulanovich, G. and Mäusezahl, D. and Hartinger, S. M.. (2022) Whole-genome characterisation of ESBL-producing E. coli isolated from drinking water and dog faeces from rural Andean households in Peru. Antibiotics (Basel), 11 (5). p. 692.

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Abstract

E. coli that produce extended-spectrum beta-lactamases (ESBLs) are major multidrug-resistant bacteria. In Peru, only a few reports have characterised the whole genome of ESBL enterobacteria. We aimed to confirm the identity and antimicrobial resistance (AMR) profile of two ESBL isolates from dog faeces and drinking water of rural Andean households and determine serotype, phylogroup, sequence type (ST)/clonal complex (CC), pathogenicity, virulence genes, ESBL genes, and their plasmids. To confirm the identity and AMR profiles, we used the VITEK((R))2 system. Whole-genome sequencing (WGS) and bioinformatics analysis were performed subsequently. Both isolates were identified as E. coli, with serotypes -:H46 and O9:H10, phylogroups E and A, and ST/CC 5259/- and 227/10, respectively. The isolates were ESBL-producing, carbapenem-resistant, and not harbouring carbapenemase-encoding genes. Isolate 1143 ST5259 harboured the astA gene, encoding the EAST1 heat-stable toxin. Both genomes carried ESBL genes (blaEC-15, blaCTX-M-8, and blaCTX-M-55). Nine plasmids were detected, namely IncR, IncFIC(FII), IncI, IncFIB(AP001918), Col(pHAD28), IncFII, IncFII(pHN7A8), IncI1, and IncFIB(AP001918). Finding these potentially pathogenic bacteria is worrisome given their sources and highlights the importance of One-Health research efforts in remote Andean communities.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
UniBasel Contributors:Venkatesh, Apoorva and Mäusezahl, Daniel and Hartinger, Stella
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2079-6382 (Print)2079-6382 (Linking)
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:27 Dec 2022 12:43
Deposited On:27 Dec 2022 12:43

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