Chami, Mohamed and Franke, Barbara and Mayans, Olga and Kreplak, Laurent. (2011) Assembly of a protein "brush" by end-grafting titin fragments to liposomes. Journal of bioscience and bioengineering, 112 (2). pp. 178-179.
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Official URL: https://edoc.unibas.ch/88849/
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Abstract
Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel) 05 Faculty of Science > Departement Biozentrum > Services Biozentrum > BioEM Lab (Chami) |
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| UniBasel Contributors: | Chami, Mohamed |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| ISSN: | 1347-4421 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 19 Jul 2022 13:42 |
| Deposited On: | 19 Jul 2022 13:42 |
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