Photoaffinity capture compounds to profile the Magic Spot Nucleotide interactomes

Haas, Thomas M. and Laventie, Benoit-Joseph and Lagies, Simon and Harter, Caroline and Prucker, Isabel and Ritz, Danilo and Batcha, Raspudin Saleem and Qiu, Danye and Hüttel, Wolfgang and Andexer, Jennifer and Kammerer, Bernd and Jenal, Urs and Jessen, Henning Jacob. (2022) Photoaffinity capture compounds to profile the Magic Spot Nucleotide interactomes. Angewandte Chemie International Edition, 61 (22). e202201731.

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Official URL: https://edoc.unibas.ch/88125/

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Magic Spot Nucleotides (MSN) regulate the stringent response, a highly conserved bacterial stress adaptation mechanism, enabling survival under adverse external challenges. In times of antibiotic crisis, a detailed understanding of stringent response is essential, as potentially new targets for pharmacological intervention could be identified. In this study, we delineate the MSN interactome in Escherichia coli and Salmonella typhimurium applying a family of trifunctional photoaffinity capture compounds. We introduce MSN probes covering diverse phosphorylation pattern, such as pppGpp, ppGpp, and pGpp. Our chemical proteomics approach provides datasets of putative MSN receptors both from cytosolic and membrane fractions that, unveil new MSN targets. We find, that the activity of the non-Nudix hydrolase ApaH is potently inhibited by pppGpp, which itself is converted to pGpp by ApaH. The capture compounds described herein will be useful to identify MSN interactomes across bacterial species.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal)
UniBasel Contributors:Jenal, Urs and Laventie, Benoit-Joseph
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Jun 2022 15:07
Deposited On:01 Jun 2022 15:07

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