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Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation

Moreno-Gámez, Stefany and Dal Co, Alma and van Vliet, Simon and Ackermann, Martin. (2021) Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation. In: Bacterial Persistence: Methods and Protocols. New York, NY, pp. 107-124.

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Official URL: https://edoc.unibas.ch/87994/

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Abstract

Nutrient limitationNutrient limitation is one of the most common triggers of antibiotic tolerance and persistence. Here, we present two microfluidicMicrofluidicssetups to study how spatial and temporal variation in nutrient availability lead to increased survival of bacteria to antibiotics. The first setup is designed to mimic the growth dynamics of bacteria in spatially structured populations (e.g., biofilmsBiofilms) and can be used to study how spatial gradients in nutrient availability, created by the collective metabolic activity of a population, increase antibiotic tolerance. The second setup captures the dynamics of feast-and-famine cycles that bacteria recurrently encounter in nature, and can be used to study how phenotypic heterogeneity in growth resumption after starvation increases survival of clonal bacterial populations. In both setups, the growth rates and metabolic activity of bacteria can be measured at the single-cellSingle-cell level. This is useful to build a mechanistic understanding of how spatiotemporal variation in nutrient availability triggers bacteria to enter phenotypic states that increase their tolerance to antibiotics.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher) > Microbial Systems Ecology (van Vliet)
UniBasel Contributors:van Vliet, Simon
Item Type:Book Section, refereed
Book Section Subtype:Further Contribution in a Book
Publisher:Springer US
ISBN:978-1-0716-1621-5
Note:Publication type according to Uni Basel Research Database: Book item
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Last Modified:09 Mar 2022 11:53
Deposited On:09 Mar 2022 11:53

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