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Elucidating Drought-Tolerance Mechanisms in Plant Roots through 1H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques

Honeker, Linnea K. and Hildebrand, Gina A. and Fudyma, Jane D. and Daber, L. Erik and Hoyt, David and Flowers, Sarah E. and Gil-Loaiza, Juliana and Kübert, Angelika and Bamberger, Ines and Anderton, Christopher R. and Cliff, John and Leichty, Sarah and Amini Tabrizi, Roya and Kreuzwieser, Jürgen and Shi, Lingling and Bai, Xuejuan and Velickovic, Dusan and Dippold, Michaela A. and Ladd, S. Nemiah and Werner, Christiane and Meredith, Laura K. and Tfaily, Malak M.. (2022) Elucidating Drought-Tolerance Mechanisms in Plant Roots through 1H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques. Environmental science and technology, 56 (3). pp. 2021-2032.

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Official URL: https://edoc.unibas.ch/87824/

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Abstract

As direct mediators between plants and soil, roots play an important role in metabolic responses to environmental stresses such as drought, yet these responses are vastly uncharacterized on a plant-specific level, especially for co-occurring species. Here, we aim to examine the effects of drought on root metabolic profiles and carbon allocation pathways of three tropical rainforest species by combining cutting-edge metabolomic and imaging technologies in an in situ position-specific 13 C-pyruvate root-labeling experiment. Further, washed (rhizosphere-depleted) and unwashed roots were examined to test the impact of microbial presence on root metabolic pathways. Drought had a species-specific impact on the metabolic profiles and spatial distribution in Piper sp. and Hibiscus rosa sinensis roots, signifying different defense mechanisms; Piper sp. enhanced root structural defense via recalcitrant compounds including lignin, while H. rosa sinensis enhanced biochemical defense via secretion of antioxidants and fatty acids. In contrast, Clitoria fairchildiana , a legume tree, was not influenced as much by drought but rather by rhizosphere presence where carbohydrate storage was enhanced, indicating a close association with symbiotic microbes. This study demonstrates how multiple techniques can be combined to identify how plants cope with drought through different drought-tolerance strategies and the consequences of such changes on below-ground organic matter composition.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften
05 Faculty of Science > Departement Umweltwissenschaften > Geowissenschaften > Geochemie Stoffkreisläufe (Lehmann)
UniBasel Contributors:Ladd, Sarah Nemiah
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:0194-0287
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:17 Feb 2022 12:21
Deposited On:17 Feb 2022 12:21

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