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Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting

Reck, Jennifer and Beuret, Nicole and Demirci, Erhan and Prescianotto-Baschong, Cristina and Spiess, Martin. (2022) Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting. Life science alliance, 5 (5). e202101279.

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Official URL: https://edoc.unibas.ch/87736/

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Abstract

Unlike constitutively secreted proteins, peptide hormones are stored in densely packed secretory granules, before regulated release upon stimulation. Secretory granules are formed at the TGN by self-aggregation of prohormones as functional amyloids. The nonapeptide hormone vasopressin, which forms a small disulfide loop, was shown to be responsible for granule formation of its precursor in the TGN as well as for toxic fibrillar aggregation of unfolded mutants in the ER. Several other hormone precursors also contain similar small disulfide loops suggesting their function as a general device to mediate aggregation for granule sorting. To test this hypothesis, we studied the capacity of small disulfide loops of different hormone precursors to mediate aggregation in the ER and the TGN. They indeed induced ER aggregation in Neuro-2a and COS-1 cells. Fused to a constitutively secreted reporter protein, they also promoted sorting into secretory granules, enhanced stimulated secretion, and increased Lubrol insolubility in AtT20 cells. These results support the hypothesis that small disulfide loops act as novel signals for sorting into secretory granules by self-aggregation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spiess)
UniBasel Contributors:Spiess, Martin
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2575-1077
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:09 Mar 2022 11:45
Deposited On:09 Mar 2022 11:45

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