Determinants of the temperature adaptation of mRNA degradation

Jaquet, Vincent and Wallerich, Sandrine and Voegeli, Sylvia and Túrós, Demeter and Viloria, Eduardo C. and Becskei, Attila. (2022) Determinants of the temperature adaptation of mRNA degradation. Nucleic Acids Research, 50 (2). pp. 1092-1110.

[img] PDF - Published Version
Available under License CC BY-NC (Attribution-NonCommercial).


Official URL: https://edoc.unibas.ch/87512/

Downloads: Statistics Overview


The rate of chemical reactions increases proportionally with temperature, but the interplay of biochemical reactions permits deviations from this relation and adaptation. The degradation of individual mRNAs in yeast increased to varying degrees with temperature. We examined how these variations are influenced by the translation and codon composition of mRNAs. We developed a method that revealed the existence of a neutral half-life above which mRNAs are stabilized by translation but below which they are destabilized. The proportion of these two mRNA subpopulations remained relatively constant under different conditions, even with slow cell growth due to nutrient limitation, but heat shock reduced the proportion of translationally stabilized mRNAs. At the same time, the degradation of these mRNAs was partially temperature-compensated through Upf1, the mediator of nonsense-mediated decay. Compensation was also promoted by some asparagine and serine codons, whereas tyrosine codons promote temperature sensitization. These codons play an important role in the degradation of mRNAs encoding key cell membrane and cell wall proteins, which promote cell integrity.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Synthetic Microbiology (Becskei)
UniBasel Contributors:Becskei, Attila
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Oxford University Press
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
edoc DOI:
Last Modified:09 Mar 2022 10:24
Deposited On:09 Mar 2022 10:24

Repository Staff Only: item control page