edoc

B cell mediated immune response in chronic viral infection studied through immunoglobulin engineering

Florova, Marianna. B cell mediated immune response in chronic viral infection studied through immunoglobulin engineering. 2021, Doctoral Thesis, University of Basel, Faculty of Science.

[img] PDF
Restricted to Repository staff only until 31 November 2022.

17Mb

Official URL: https://edoc.unibas.ch/87422/

Downloads: Statistics Overview

Abstract

Protective neutralizing antibodies against chronic viral pathogens arise late after the onset of the infection and are not trivial to elicit with immunization. Both in the polyclonal B cell repertoire and upon characterizing the newly generated neutralizing antibody, KL25 heavy and light chain knock-in mice, expressing B cells with high affinity to the lymphocytic choriomeningitis virus (LCMV) glycoprotein (GP), we observed an IgM molecule downregulation on the surface of the naïve B cells that was indirectly proportional to the LCMV GP binding. IgMlow IgD+ phenotype did not negatively affect the activation potential, adaptation and antibody production upon germinal center entry of the GP-binding B cells in chronic viral infection; however, RAG2-dependent negative selection during light-chain receptor editing renders glycoprotein binding specificity a rare phenomenon in the peripheral B cell repertoire.
Advisors:Pinschewer, Daniel and Pieters, Jean and Pircher, Hanspeter
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Division of Medical Microbiology > Experimental Virology (Pinschewer)
UniBasel Contributors:Pinschewer, Daniel and Pieters, Jean
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:14596
Thesis status:Complete
Number of Pages:168
Language:English
Identification Number:
  • urn: urn:nbn:ch:bel-bau-diss145966
edoc DOI:
Last Modified:15 Feb 2022 10:57
Deposited On:08 Feb 2022 11:08

Repository Staff Only: item control page