Florova, Marianna. B cell mediated immune response in chronic viral infection studied through immunoglobulin engineering. 2021, Doctoral Thesis, University of Basel, Faculty of Science.
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Official URL: https://edoc.unibas.ch/87422/
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Abstract
Protective neutralizing antibodies against chronic viral pathogens arise late after the onset of the infection and are not trivial to elicit with immunization. Both in the polyclonal B cell repertoire and upon characterizing the newly generated neutralizing antibody, KL25 heavy and light chain knock-in mice, expressing B cells with high affinity to the lymphocytic choriomeningitis virus (LCMV) glycoprotein (GP), we observed an IgM molecule downregulation on the surface of the naïve B cells that was indirectly proportional to the LCMV GP binding. IgMlow IgD+ phenotype did not negatively affect the activation potential, adaptation and antibody production upon germinal center entry of the GP-binding B cells in chronic viral infection; however, RAG2-dependent negative selection during light-chain receptor editing renders glycoprotein binding specificity a rare phenomenon in the peripheral B cell repertoire.
Advisors: | Pinschewer, Daniel and Pieters, Jean and Pircher, Hanspeter |
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Faculties and Departments: | 03 Faculty of Medicine > Departement Biomedizin > Division of Medical Microbiology > Experimental Virology (Pinschewer) |
UniBasel Contributors: | Pinschewer, Daniel and Pieters, Jean |
Item Type: | Thesis |
Thesis Subtype: | Doctoral Thesis |
Thesis no: | 14596 |
Thesis status: | Complete |
Number of Pages: | 168 |
Language: | English |
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edoc DOI: | |
Last Modified: | 01 Dec 2022 02:30 |
Deposited On: | 08 Feb 2022 11:08 |
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