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Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH

Wimmi, Stephan and Balinovic, Alexander and Jeckel, Hannah and Selinger, Lisa and Lampaki, Dimitrios and Eisemann, Emma and Meuskens, Ina and Linke, Dirk and Drescher, Knut and Endesfelder, Ulrike and Diepold, Andreas. (2021) Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH. Nature communications, 12 (1). p. 1625.

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Official URL: https://edoc.unibas.ch/87210/

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Abstract

Many bacterial pathogens use a type III secretion system (T3SS) to manipulate host cells. Protein secretion by the T3SS injectisome is activated upon contact to any host cell, and it has been unclear how premature secretion is prevented during infection. Here we report that in the gastrointestinal pathogens Yersinia enterocolitica and Shigella flexneri, cytosolic injectisome components are temporarily released from the proximal interface of the injectisome at low external pH, preventing protein secretion in acidic environments, such as the stomach. We show that in Yersinia enterocolitica, low external pH is detected in the periplasm and leads to a partial dissociation of the inner membrane injectisome component SctD, which in turn causes the dissociation of the cytosolic T3SS components. This effect is reversed upon restoration of neutral pH, allowing a fast activation of the T3SS at the native target regions within the host. These findings indicate that the cytosolic components form an adaptive regulatory interface, which regulates T3SS activity in response to environmental conditions.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:natureportfolio
ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:22 Jan 2022 15:44
Deposited On:22 Jan 2022 15:44

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