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Sialoglycan – Siglec axis in the modulation of dendritic cells functions

Wang, Jinyu. Sialoglycan – Siglec axis in the modulation of dendritic cells functions. 2021, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: https://edoc.unibas.ch/87090/

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Abstract

Interactions between sialylated glycans and Siglec receptors have been recently described as potential new immune checkpoint that can be targeted to improve anticancer immunity. Myeloid cells have been reported to express a wide range of different Siglecs, however their expression and functions on cancer-associated dendritic cells (DCs) were not fully characterized. We found that classical conventional DCs (cDCs) from cancer patient samples have a high expression of several inhibitory Siglecs including Siglec-7, Siglec-9 and Siglec-10. In subcutaneous murine tumor models, we also found an upregulation of the inhibitory Siglec-E receptor on cancer-associated cDCs. DC cell lines and BMDCs with expression of these inhibitory Siglecs showed impaired maturation states on transcriptome and protein level. Furthermore, ablation of these inhibitory Siglecs from DCs enhanced their capability to prime antigen specific T cells and induce proliferation. Our work provides a deeper understanding of the influence of inhibitory Siglecs on DCs, and reveals a potential new target to improve cancer immunotherapy.
Advisors:Läubli, Heinz and Christofori, Gerhard M. and von Gunten, Stephan
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Cellular Immunotherapy (Läubli)
03 Faculty of Medicine > Departement Biomedizin > Division of Biochemistry and Genetics > Tumor Biology (Christofori)
UniBasel Contributors:Christofori, Gerhard M.
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:14607
Thesis status:Complete
Number of Pages:105
Language:English
Identification Number:
  • urn: urn:nbn:ch:bel-bau-diss146075
edoc DOI:
Last Modified:15 Feb 2022 10:53
Deposited On:10 Feb 2022 15:32

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