Shyp, Viktoriya and Dubey, Badri Nath and Böhm, Raphael and Hartl, Johannes and Nesper, Jutta and Vorholt, Julia A. and Hiller, Sebastian and Schirmer, Tilman and Jenal, Urs. (2021) Reciprocal growth control by competitive binding of nucleotide second messengers to a metabolic switch in Caulobacter crescentus. Nature Microbiology, 6 (1). pp. 59-72.
![]() |
PDF
Restricted to Repository staff only 11Mb |
Official URL: https://edoc.unibas.ch/86783/
Downloads: Statistics Overview
Abstract
Bacteria use small signalling molecules such as (p)ppGpp or c-di-GMP to tune their physiology in response to environmental changes. It remains unclear whether these regulatory networks operate independently or whether they interact to optimize bacterial growth and survival. We report that (p)ppGpp and c-di-GMP reciprocally regulate the growth of Caulobacter crescentus by converging on a single small-molecule-binding protein, SmbA. While c-di-GMP binding inhibits SmbA, (p)ppGpp competes for the same binding site to sustain SmbA activity. We demonstrate that (p)ppGpp specifically promotes Caulobacter growth on glucose, whereas c-di-GMP inhibits glucose consumption. We find that SmbA contributes to this metabolic switch and promotes growth on glucose by quenching the associated redox stress. The identification of an effector protein that acts as a central regulatory hub for two global second messengers opens up future studies on specific crosstalk between small-molecule-based regulatory networks.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development 05 Faculty of Science > Departement Biozentrum > Infection Biology 05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal) 05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal) 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller) |
---|---|
UniBasel Contributors: | Schirmer, Tilman and Hiller, Sebastian and Jenal, Urs |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Nature Publishing Group |
e-ISSN: | 2058-5276 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
|
edoc DOI: | |
Last Modified: | 06 Oct 2022 03:10 |
Deposited On: | 03 Mar 2022 15:32 |
Repository Staff Only: item control page