Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions

Tocchini, Cristina and Rohner, Michèle and Guerard, Laurent and Ray, Poulomi and Von Stetina, Stephen E. and Mango, Susan E.. (2021) Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions. Development, 148 (24). p. 200027.

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Official URL: https://edoc.unibas.ch/86637/

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mRNA localization is an evolutionarily widespread phenomenon that can facilitate subcellular protein targeting. Extensive work has focused on mRNA targeting through 'zip-codes' within untranslated regions (UTRs), whereas much less is known about translation-dependent cues. Here, we examine mRNA localization in Caenorhabditis elegans embryonic epithelia. From an smFISH-based survey, we identified mRNAs associated with the cell membrane or cortex, and with apical junctions in a stage- and cell type-specific manner. Mutational analyses for one of these transcripts, dlg-1/discs large, revealed that it relied on a translation-dependent process and did not require its 5' or 3' UTRs. We suggest a model in which dlg-1 transcripts are co-translationally localized with the nascent protein: first the translating complex goes to the cell membrane using sequences located at the C-terminal/3' end, and then apically using N-terminal/5' sequences. These studies identify a translation-based process for mRNA localization within developing epithelia and determine the necessary cis-acting sequences for dlg-1 mRNA targeting.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango)
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Imaging Core Facility (Biehlmaier)
UniBasel Contributors:Guerard, Laurent and Tocchini, Cristina and Rohner, Michèle and Ray, Poulomi and Mango, Susan Elizabeth
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Company of Biologists
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:02 May 2023 09:01
Deposited On:03 Mar 2022 14:52

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