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Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases

Maffei, Enea and Fino, Cinzia and Harms, Alexander. (2021) Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases. In: Bacterial Persistence, 2357. Cham, pp. 23-40.

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Official URL: https://edoc.unibas.ch/84674/

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Abstract

Antibiotic tolerance and persistence allow bacteria to survive lethal doses of antibiotic drugs in the absence of genetic resistance. Despite the urgent need to address these phenomena as a cause of clinical antibiotic treatment failure, studies on antibiotic tolerance and persistence are notorious for contradictory and inconsistent findings. Many of these problems are likely caused by differences in the methodology used to study antibiotic tolerance and persistence in the laboratory. Standardized experimental procedures would therefore greatly promote research in this field by facilitating the integrated analysis of results obtained by different research groups. Here, we present a robust and adaptable methodology to study antibiotic tolerance/persistence in broth cultures of Escherichia coli and Pseudomonas aeruginosa . The hallmark of this methodology is that the formation and disappearance of antibiotic-tolerant cells is recorded throughout all bacterial growth phases from lag after inoculation over exponential growth into early and then late stationary phase. In addition, all relevant experimental conditions are rigorously controlled to obtain highly reproducible results. We anticipate that this methodology will promote research on antibiotic tolerance and persistence by enabling a deeper view at the growth-dependent dynamics of this phenomenon and by contributing to the standardization or at least comparability of experimental procedures used in the field.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal) > Molecular Microbiology (Harms)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal) > Molecular Microbiology (Harms)
UniBasel Contributors:Maffei, Enea and Fino, Cinzia and Harms, Alexander
Item Type:Book Section
Book Section Subtype:Further Contribution in a Book
Publisher:Springer Nature
ISBN:978-1-0716-1621-5
Series Name:Methods in Molecular Biology
Note:Publication type according to Uni Basel Research Database: Book item
Identification Number:
Last Modified:22 Nov 2021 16:55
Deposited On:22 Nov 2021 16:55

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