Probing and manipulating embryogenesis via nanoscale thermometry and temperature control

Choi, Joonhee and Zhou, Hengyun and Landig, Renate and Wu, Hai-Yin and Yu, Xiaofei and Von Stetina, Stephen E. and Kucsko, Georg and Mango, Susan E. and Needleman, Daniel J. and Samuel, Aravinthan D. T. and Maurer, Peter C. and Park, Hongkun and Lukin, Mikhail D.. (2020) Probing and manipulating embryogenesis via nanoscale thermometry and temperature control. Proceedings of the National Academy of Sciences of the United States of America, 117 (26). pp. 14636-14641.

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Understanding the coordination of cell-division timing is one of the outstanding questions in the field of developmental biology. One active control parameter of the cell-cycle duration is temperature, as it can accelerate or decelerate the rate of biochemical reactions. However, controlled experiments at the cellular scale are challenging, due to the limited availability of biocompatible temperature sensors, as well as the lack of practical methods to systematically control local temperatures and cellular dynamics. Here, we demonstrate a method to probe and control the cell-division timing in; Caenorhabditis elegans; embryos using a combination of local laser heating and nanoscale thermometry. Local infrared laser illumination produces a temperature gradient across the embryo, which is precisely measured by in vivo nanoscale thermometry using quantum defects in nanodiamonds. These techniques enable selective, controlled acceleration of the cell divisions, even enabling an inversion of division order at the two-cell stage. Our data suggest that the cell-cycle timing asynchrony of the early embryonic development in; C. elegans; is determined independently by individual cells rather than via cell-to-cell communication. Our method can be used to control the development of multicellular organisms and to provide insights into the regulation of cell-division timings as a consequence of local perturbations.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango)
UniBasel Contributors:Mango, Susan Elizabeth
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:07 Sep 2021 14:11
Deposited On:07 Sep 2021 14:11

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