Hard X-ray microtomography of Zebrafish larvae

Osterwalder, Melissa and Bolten, Jan Stephan and Rodgers, Griffin and Humbel, Mattia and Schulz, Georg and Tanner, Christine and Huwyler, Jörg and Müller, Bert. (2020) Hard X-ray microtomography of Zebrafish larvae. Spiedigitallibrary, Conference Proceedings of SPIE, 11886. p. 118864.

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Official URL: https://edoc.unibas.ch/84156/

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Hard X-ray micro computed tomography can be used for three-dimensional histological phenotyping of zebrafish embryos down to 1 µm or below without the need for staining or physical slicing. Current advances in ze- brafish embryo imaging, however, mostly rely on synchrotron radiation sources or highly advanced laboratory sources, which despite their evident strengths with regard to their beam properties and the implementation of phase contrast imaging techniques, lack accessibility. Therefore, we evaluated the performance of a conventional SkyScan 1275 laboratory µCT scanner in absorption contrast mode for the visualization of anatomical features in ethanol- and paraffin-embedded zebrafish embryos. We compare our results to readily available synchrotron data where 35 anatomical structures were identified. Despite having a more than ten times larger voxel length, approximately two thirds of the features could also be determined with laboratory microtomography. This could allow to monitor morphological changes during development or disease progression on large sample numbers, enabling the performance of preclinical studies in a local laboratory.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedical Engineering > Imaging and Computational Modelling > Biomaterials Science Center (Müller)
05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmaceutical Technology (Huwyler)
UniBasel Contributors:Huwyler, Jörg and Bolten, Jan Stephan and Osterwalder, Melissa and Rodgers, Griffin and Schulz, Georg and Tanner, Christine and Müller, Bert
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Aug 2021 09:11
Deposited On:31 Aug 2021 09:11

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