RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae

Peschek, Nikolai and Herzog, Roman and Singh, Praveen K. and Sprenger, Marcel and Meyer, Fabian and Fröhlich, Kathrin S. and Schröger, Luise and Bramkamp, Marc and Drescher, Knut and Papenfort, Kai. (2020) RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae. Nature Communications, 11. p. 6067.

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Vibrio cholerae, the cause of cholera disease, exhibits a characteristic curved rod morphology, which promotes infectivity and motility in dense hydrogels. Periplasmic protein CrvA determines cell curvature in V. cholerae, yet the regulatory factors controlling CrvA are unknown. Here, we discover the VadR small RNA (sRNA) as a post-transcriptional inhibitor of the crvA mRNA. Mutation of vadR increases cell curvature, whereas overexpression has the inverse effect. We show that vadR transcription is activated by the VxrAB two-component system and triggered by cell-wall-targeting antibiotics. V. cholerae cells failing to repress crvA by VadR display decreased survival upon challenge with penicillin G indicating that cell shape maintenance by the sRNA is critical for antibiotic resistance. VadR also blocks the expression of various key biofilm genes and thereby inhibits biofilm formation in V. cholerae. Thus, VadR is an important regulator for synchronizing peptidoglycan integrity, cell shape, and biofilm formation in V. cholerae.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:25 Jun 2021 09:14
Deposited On:25 Jun 2021 09:14

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