Division of Labor during Biofilm Matrix Production

Dragoš, Anna and Kiesewalter, Heiko and Martin, Marivic and Hsu, Chih-Yu and Hartmann, Raimo and Wechsler, Tobias and Eriksen, Carsten and Brix, Susanne and Drescher, Knut and Stanley-Wall, Nicola and Kümmerli, Rolf and Kovács, Ákos T.. (2018) Division of Labor during Biofilm Matrix Production. Current Biology, 28 (12). pp. 1903-1913.e5.

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Official URL: https://edoc.unibas.ch/83576/

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Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation. Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations or genetically distinct lineages cooperate with each other by performing complementary tasks. Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production. We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis. In this species, biofilm matrix consists of two major components, exopolysaccharides (EPSs) and TasA. We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS producers, and generalists, which produce both EPSs and TasA. This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPSs and TasA. The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA producers, corresponding exactly to the population composition where group productivity is maximized. Using individual-based modeling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Jun 2021 10:23
Deposited On:23 Jun 2021 10:23

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