Structural dynamics of RbmA governs plasticity of Vibrio cholerae biofilms

Fong, Jiunn C. N. and Rogers, Andrew and Michael, Alicia K. and Parsley, Nicole C. and Cornell, William-Cole and Lin, Yu-Cheng and Singh, Praveen K. and Hartmann, Raimo and Drescher, Knut and Vinogradov, Evgeny and Dietrich, Lars E. P. and Partch, Carrie L. and Yildiz, Fitnat H.. (2017) Structural dynamics of RbmA governs plasticity of Vibrio cholerae biofilms. eLife, 6. e26163.

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Official URL: https://edoc.unibas.ch/83571/

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Biofilm formation is critical for the infection cycle of; Vibrio cholerae. Vibrio; exopolysaccharides (VPS) and the matrix proteins RbmA, Bap1 and RbmC are required for the development of biofilm architecture. We demonstrate that RbmA binds VPS directly and uses a binary structural switch within its first fibronectin type III (FnIII-1) domain to control RbmA structural dynamics and the formation of VPS-dependent higher-order structures. The structural switch in FnIII-1 regulates interactions in trans with the FnIII-2 domain, leading to open (monomeric) or closed (dimeric) interfaces. The ability of RbmA to switch between open and closed states is important for; V. cholerae; biofilm formation, as RbmA variants with switches that are locked in either of the two states lead to biofilms with altered architecture and structural integrity.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Jun 2021 09:34
Deposited On:23 Jun 2021 09:34

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