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Architectural transitions in Vibrio cholerae biofilms at single-cell resolution

Drescher, Knut and Dunkel, Jörn and Nadell, Carey D. and van Teeffelen, Sven and Grnja, Ivan and Wingreen, Ned S. and Stone, Howard A. and Bassler, Bonnie L.. (2016) Architectural transitions in Vibrio cholerae biofilms at single-cell resolution. Proceedings of the National Academy of Sciences of the United States of America, 113 (14). E2066-E2072.

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Abstract

Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth. Bacterial biofilms display remarkable changes during their development from initial attachment to maturity, yet the cellular architecture that gives rise to collective biofilm morphology during growth is largely unknown. Here, we use high-resolution optical microscopy to image all individual cells in Vibrio cholerae biofilms at different stages of development, including colonies that range in size from 2 to 4,500 cells. From these data, we extracted the precise 3D cellular arrangements, cell shapes, sizes, and global morphological features during biofilm growth on submerged glass substrates under flow. We discovered several critical transitions of the internal and external biofilm architectures that separate the major phases of V. cholerae biofilm growth. Optical imaging of biofilms with single-cell resolution provides a new window into biofilm formation that will prove invaluable to understanding the mechanics underlying biofilm development.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
ISSN:0027-8424
e-ISSN:1091-6490
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:23 Jun 2021 09:10
Deposited On:23 Jun 2021 09:10

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