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Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography

Klena, Nikolai and Le Guennec, Maeva and Tassin, Anne-Marie and van den Hoek, Hugo and Erdmann, Philipp S. and Schaffer, Miroslava and Geimer, Stefan and Aeschlimann, Gabriel and Kovacik, Lubomir and Sadian, Yashar and Goldie, Kenneth N. and Stahlberg, Henning and Engel, Benjamin D. and Hamel, Virginie and Guichard, Paul. (2020) Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography. The EMBO journal, 39 (22). e106246.

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Official URL: https://edoc.unibas.ch/81967/

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Abstract

Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In both Paramecium and Chlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25 nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis.
Faculties and Departments:05 Faculty of Science
UniBasel Contributors:Goldie, Kenneth N.
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1460-2075
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:15 Feb 2021 09:42
Deposited On:15 Feb 2021 09:42

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